One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase
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چکیده
منابع مشابه
Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold.
DNA polymerases are involved in all DNA synthesis that occurs in nature. Furthermore, DNA polymerases are the workhorses in numerous important molecular biological core technologies like the widely applied polymerase chain reaction (PCR), cDNA cloning, genome sequencing, and diagnostics based on nucleic acids. In each of these processes the DNA polymerase requires a DNA strand as a template for...
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DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and...
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The highly thermostable DNA polymerase from Thermus aquaticus (Taq) is ideal for both manual and automated DNA sequencing because it is fast, highly processive, has little or no 3'-exonuclease activity, and is active over a broad range of temperatures. Sequencing protocols are presented that produce readable extension products greater than 1000 bases having uniform band intensities. A combinati...
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ژورنال
عنوان ژورنال: Biotechnology Journal
سال: 2010
ISSN: 1860-6768
DOI: 10.1002/biot.200900200